Fig. 4: Hepcidin induction in the liver after a cerebral ischemic insult. | Experimental & Molecular Medicine

Fig. 4: Hepcidin induction in the liver after a cerebral ischemic insult.

From: Hepatocyte activation and liver injury following cerebral ischemia promote HMGB1-mediated hepcidin upregulation in hepatocytes and regulation of systemic iron levels

Fig. 4

Hepcidin protein (a) and hepcidin mRNA (b) levels were determined in liver samples obtained at 3, 6, 12, 24, 48, 72, and 96 h after MCAO using ELISA and RT‒qPCR, respectively, and the results are presented as the means ± SEMs (n = 4). *p < 0.05 and **p < 0.01 compared with the sham control group. Liver tissue sections from sham controls (c–d) and the MCAO group (e–g) were prepared at 12 h post-MCAO and subjected to immunohistochemistry using an anti-hepcidin antibody, followed by counterstaining using H&E. h The staining intensity was measured using ImageJ software, and the quantified results are presented as the means ± SEMs (n = 18 from 3 animals). **p < 0.01 compared with the sham control groups Arrows indicate hepcidin immunoreactivity in hepatocytes, and arrowheads indicate hepcidin immunoreactivity in Kupffer cells. The scale bars in c and e represent 50 µm, and those in d, f, and g represent 20 µm.

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