Fig. 8: Cbx4 mediates K1111 SUMOylation of BRD4. | Experimental & Molecular Medicine

Fig. 8: Cbx4 mediates K1111 SUMOylation of BRD4.

From: Cbx4 SUMOylates BRD4 to regulate the expression of inflammatory cytokines in post-traumatic osteoarthritis

Fig. 8

a Potential SUMOylation sites in the BRD4 protein predicted via the UniProt database (https://www.uniprot.org) and the GPS-SUMO database (https://sumo.biocuckoo.cn/). b Relative HA expression levels in each BRD4-mutated overexpression group determined by western blotting. c Relative protein expression of Cbx4 in the synovial fibroblasts of the rats was determined via western blotting. d The protein contents of SUMO1 and SUMO2/3 in each BRD4 (wild-type or mutant) and HA-fused overexpressing cell subjected to inflammatory induction by HMGB1 were determined via co-IP with an anti-HA antibody. e Ubiquitination levels of the BRD4-HA fusion protein determined in the same cell samples as in d by the co-IP method. f Ubiquitination level of the BRD4-HA fusion protein determined by co-IP after 2-D08 treatment. Drug dosages and durations: HMGB1 (10 ng/mL) and 2-D08 (5 nM) for 24 h.

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