Fig. 9: Inhibition of Cbx4 alleviates ACLT-induced knee arthritis in rats. | Experimental & Molecular Medicine

Fig. 9: Inhibition of Cbx4 alleviates ACLT-induced knee arthritis in rats.

From: Cbx4 SUMOylates BRD4 to regulate the expression of inflammatory cytokines in post-traumatic osteoarthritis

Fig. 9

a Schematic diagram of the animal experimental procedure. b Rat weight loss data. c OARSI scores of the rats. d Three-dimensional imaging of rat knee tissues via micro-CT scanning. e Representative images of Safranin O-fast green and Alcian blue staining of cartilage tissues from rats. f Transcriptional activities of IL-1β, TNF-α and IL-6 evaluated by ChIP/qPCR. g Relative mRNA expression levels of IL-1β, TNF-α and IL-6 were determined by qPCR. Ubiquitination (h) and SUMOylation (i) levels of BRD4 in the synovial tissues of rats determined by co-IP. Drug dosage, frequency, and duration of use: UNC3866 (5 mg/mL, 50 μL) was administered once daily for one week. In c, f, and g, n = 5, one-way ANOVA. *P < 0.05; **P < 0.01; ***P < 0.005.

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