Fig. 4: Nonobese phenotype assessments of heteroplasmic gene-knockout mutant mice.
From: Comprehensive phenotypic assessment of nonsense mutations in mitochondrial ND5 in mice
a Representative images of nonobese mutant mice. b Average body weights of WT (n = 5) and obese mutant mice (n = 10). Error bars indicate the s.e.m. for biologically independent mice (n ≥ 5). The exact p values for 7, 8, 9, 10, 11, 12, 13, and 14 weeks were 0.27, 0.37, 0.44, 0.23, 0.21, 0.15, 0.21, and 0.23, respectively (n.s., not significant, using Student’s two-tailed t test). c Mean body weight, fat mass, and fat/body weight ratio in WT mice (n = 3) and mutant-non-Ob mice (n = 3) measured by dual-energy X-ray absorptiometry. The error bars indicate the s.e.m. for biologically independent mice (n = 3). The exact p values of body weight, fat mass, and the fat mass/body weight ratio were 0.13, 0.28, and 0.057, respectively (n.s., not significant; Student’s two-tailed t test was used). d Images of perirenal fat, abdominal fat, and epidydimal fat tissues from WT and Mutant-non-Ob mice. Each grid in the image is 5 mm long. The measured weights of the fat tissues are shown on the right. The error bars indicate the s.e.m. for biologically independent mice (n = 3). The exact p values were 0.71, 0.49, and 0.68 for abdominal fat, epidydimal fat, and perirenal fat, respectively (n.s., not significant; Student’s two-tailed t test was used). e (Left) Microscopy image of perirenal fat via H&E at ×200 magnification. (Right) Measurements of the sizes of perirenal fat cells. Lines represent the mean with s.e.m. The exact p value was 2.0E-18 (***p < 0.001 using Student’s two-tailed t test). f Transmission electron micrographs of mitochondria in white adipose tissues of WT and nonobese mutant mice. g Analysis of ATP synthesis in white adipose tissues of WT (n = 3) and mutant mice (n = 4). The error bars indicate the SEMs for biologically independent samples (n ≥ 3). The exact p value was 0.0002 (***p < 0.001 using Student’s two-tailed t test). h Average body weights of WT (n = 5) and non-Ob-phenotype mutant mice (n = 4) after high-fat feeding (arrow). Error bars indicate the s.e.m. for biologically independent mice (n ≥ 3). The exact p values for 6, 7, 8, 9, and 10 weeks were 0.77, 0.14, 0.0061, 0.041, and 0.0069, respectively (*p < 0.05, **p < 0.01, and ***p < 0.001 according to a two-tailed Student’s t test).
