Fig. 6: IAMP29 treatment increases the PKM2 mRNA expression level and activates the NLRP3 inflammasome via PKR signaling.
From: The inflammasome-activating poxvirus peptide IAMP29 promotes antimicrobial and anticancer responses
Dose (a)- or time (b)-dependent expression of PKM1 and PKM2 in IAMP29-treated human monocytes. c Western blots showing the levels of PKM2 in the lysates of IAMP29-treated human monocytes or PMA-differentiated THP-1 cells. d, e The ECAR in PMA-differentiated THP-1 cells treated with IAMP29 (25 μg/mL for 18 h) after LPS stimulation. f Hif1a mRNA expression in IAMP29-treated murine BMDMs (25 μg/mL for 3, 6, and 18 h). g Pkm1 and Pkm2 mRNA expression in IAMP29-treated murine BMDMs after pretreatment with the indicated inhibitors. h IL1B and IL18 mRNA expression in A30L- or IAMP29-treated human monocytes after pretreatment with the indicated inhibitors. i Western blots showing the levels of mature IL-1β in culture supernatants and pro-IL-1β, PKR, and phospho-PKR in the lysates of IAMP29-treated monocytes cultured in the presence of the indicated inhibitors. Statistical analysis was conducted with an unpaired t-test with the Mann‒Whitney test or one-way ANOVA with Tukey’s multiple comparison test, and the results are presented as the means ± SDs. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. a.u. arbitrary unit, ns not significant.
