Fig. 7: Exendin 9–39 had no effect on proton-induced TRPV1 currents or calcium influx in CHO K1 cells expressing human TRPV1. | Experimental & Molecular Medicine

Fig. 7: Exendin 9–39 had no effect on proton-induced TRPV1 currents or calcium influx in CHO K1 cells expressing human TRPV1.

From: GLP-1 and its derived peptides mediate pain relief through direct TRPV1 inhibition without affecting thermoregulation

Fig. 7

a Representative proton-induced inward currents evoked at pH 5.5 under control conditions (blue) or with 1 µM exendin 9–39 (blue) or 1 µM BCTC (orange) pretreatment. b Mean normalized current amplitude of TRPV1 currents (nA) after treatment with 1 µM exendin 9–39 or 1 µM BCTC compared with the control, pH 5.5 (blue) (mean ± S.E.M.). One-way ANOVA followed by Dunnett’s multiple comparison test (ns, not significant; ****p < 0.0001, compared with the control, pH 5.5). c Representative proton-induced calcium influx at pH 5.5 under control conditions (blue) or with 1 µM exendin 9–39 (blue) or 1 µM BCTC (orange) pretreatment. d Mean normalized 340/380 ratios of sequential proton-induced calcium increases (mean ± S.E.M.). One-way ANOVA followed by Dunnett’s multiple comparison test (ns, not significant; ****p < 0.0001, compared with the control, pH 5.5). ANOVA analysis of variance, BCTC N-(4-tertiarybutylphenyl)-4-(3-cholorphyridin-2-yl) tetrahydropyrazine-1(2H)-carboxamide, CHO K1 Chinese hamster ovary, S.E.M. standard error of the mean, TRPV1 transient receptor potential vanilloid 1.

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