Fig. 3: In vitro PC cells inhibit insulin secretion in MIN6 cells in a sorcin-dependent manner. | Experimental & Molecular Medicine

Fig. 3: In vitro PC cells inhibit insulin secretion in MIN6 cells in a sorcin-dependent manner.

From: Sorcin can trigger pancreatic cancer-associated new-onset diabetes through the secretion of inflammatory cytokines such as serpin E1 and CCL5

Fig. 3

a Schematic diagram of the cell experimental design. b The mRNA and c protein levels of SRI in PC cell lines and a normal pancreatic duct cell line (HPDE6). d Detection of the knockdown effect in ASPC-1 cells by immunoblotting. e Detection of insulin content in the supernatant after GSIS in MIN6 cells incubated with conditioned medium from AsPC-1 cells pretreated with NC siRNA (CM-NC-siRNA) or SRI siRNA (CM-SRI-siRNA). f Detection of insulin content in MIN6 cells incubated with CM-NC-siRNA or CM-SRI-siRNA from AsPC-1 cells by immunoblotting. g Expression of Ins mRNA in MIN6 cells incubated with different conditioned media from AsPC-1 cells. h Immunofluorescence image showing the content of insulin in MIN6 cells treated with different conditioned media from AsPC-1 cells. The expression of i Pdx1, j Mafa, k Foxo1 and l Rfx6 mRNA in MIN6 cells incubated with different conditioned medium from AsPC-1 cells. m Morphology of MIN6 cells treated with different conditioned medium from AsPC-1 cells. n Assessment of the viability of MIN6 cells treated with different conditioned medium from AsPC-1 cells via MTT assays. o Detection of apoptosis via flow cytometry in MIN6 cells treated with different conditioned medium from AsPC-1 cells. p Quantification of the percentage of apoptotic cells. Ns, not significant; *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001, means ± SD are shown. Statistical analysis was performed via Student’s t test for two groups.

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