Fig. 5: Sorcin-overexpressing PC cells release CCL5 and serpin E1 to inhibit insulin secretion in MIN6 cells. | Experimental & Molecular Medicine

Fig. 5: Sorcin-overexpressing PC cells release CCL5 and serpin E1 to inhibit insulin secretion in MIN6 cells.

From: Sorcin can trigger pancreatic cancer-associated new-onset diabetes through the secretion of inflammatory cytokines such as serpin E1 and CCL5

Fig. 5

a Human cytokine array of supernatants from PANC-1 cells treated with CM-NC-siRNA or CM-SRI-siRNA. b Quantitative analysis of downregulated components in PANC-1 cells treated with SRI siRNA in (a). c Detection of the mRNA levels of SRI and 5 downregulated cytokines by RT‒PCR in PANC-1 cells. Fresh culture media containing inflammatory factors was changed every 12 h until 72 h after MIN6 cells were seeded, and the insulin content in the supernatant after GSIS in MIN6 cells was treated with gradient concentrations of (d) CCL5 and (e) serpin E1. The phosphorylation levels of p38 in MIN6 cells incubated with different concentrations of (f) CCL5 and (g) serpin E1 at concentrations of 0, 25, 50 and 100 nM. h Detection of the phosphorylation level of the p38 pathway in MIN6 cells treated with different conditioned media from PANC-1, CFPAC-1 and AsPC-1 cells for 72 h. i Photos of nude mice following subcutaneous injections of pCDH-NC or pCDH-SRI PC cells and subsequent intratumoral injections of PBS, CCL5, or serpin E1 after tumor formation. j Fasting blood glucose detection in nude mice after absolute fasting for 24 h before sacrifice. k Fasting insulin level detection in nude mice after absolute fasting for 24 h before sacrifice. l Photos of subcutaneous tumors after surgical removal. m Quantitative results of tumor volume. n Immunofluorescence of islets in the pancreas of nude mice labeled with insulin and PDX1 proteins. Ns, not significant; *P < 0.05; **P < 0.01, means ± SD are shown. Statistical analysis was performed via Student’s t test for two groups.

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