Fig. 4: Astrocyte-specific inhibition of miRNA-382-5p reduces mitochondrial damage after TBI. | Experimental & Molecular Medicine

Fig. 4: Astrocyte-specific inhibition of miRNA-382-5p reduces mitochondrial damage after TBI.

From: Astrocyte–neuron crosstalk through extracellular vesicle-shuttled miRNA-382-5p promotes traumatic brain injury

Fig. 4

a Representative electron microscopy (EM) images showing the number of mitochondrial cristae, the matrix density, and swelling in the perilesional cortex after TBI. b TEM images showing mitochondrial crista remodeling in the perilesional cortex of the AAV-miR-382-5pi group or AAV-Ctrl group. c Fifty randomly selected mitochondria per sample were scored according to the number of mitochondrial cristae and divided into three categories: more than four cristae (Class I), two or three cristae (Class II), and one or no cristae (Class III) per mitochondrion. The proportion of mitochondria in each class was calculated (n = 6 per group, one-way ANOVA). d Fifty randomly selected mitochondria per sample were scored according to matrix density and swelling and divided into two categories: dense matrix (Class A) and swollen mitochondria with a hypodense matrix (Class B). The proportion of mitochondria in each class was calculated (n = 6 per group, one-way ANOVA). e The length of the mitochondria in each group, which is represented by the mean length of 50 randomly selected mitochondria (n = 6 per group; one-way ANOVA). The data are presented as the means ± SDs; *p < 0.05, ***p < 0.001, and NS not significant.

Back to article page