Fig. 3: Genetic and pharmacological inhibition of EPRS1 attenuates kidney function and structure in FA mice.
From: EPRS1-mediated fibroblast activation and mitochondrial dysfunction promote kidney fibrosis
a Study designed to examine the effect of genetic Eprs1 inhibition in FA mice. b, c BUN and serum creatinine levels in the three groups are indicated (n = 4–6). d Representative images of PAS, MT, and IHC staining of EPRS1 in kidney tissues (n = 4–6). e–g Tubular dilatation in the PAS-stained kidney, the fibrotic area in the MT-stained kidney, and the EPRS1-positive area were quantified via ImageJ analysis (n = 4‒6). Scale bar = 100 μm. h–k BUN levels, serum creatinine levels, creatinine clearance, and the urine protein-to-creatinine ratio (UPCR) in the five groups were measured to assess the effect of the EPRS1 inhibitor (n = 7–9). l Representative images of PAS, MT, and IHC staining of EPRS1 in kidney tissues (n = 7–9). m–o Tubular dilatation in the PAS-stained kidney, the fibrotic area in the MT-stained kidney, and the EPRS1-positive area were quantified via ImageJ analysis. The data are presented as mean ± standard deviation. Statistical data were analyzed by ANOVA with Tukey’s post hoc test. *P < 0.05, **P < 0.01, and ***P < 0.001.
