Fig. 7: Genetic and pharmacological inhibition of EPRS1 decreases p-STAT3 in FA mice.
From: EPRS1-mediated fibroblast activation and mitochondrial dysfunction promote kidney fibrosis
a Representative images of phospho-signal transducer and activator of transcription 3 (p-STAT3, brown) from an IHC-stained kidney showing the effect of genetic Eprs1 inhibition in FA mice. Scale bar = 50 μm. b The p-STAT3-positive area was quantified by ImageJ analysis (n = 4–6). c, d Representative Western blot and quantitative data of protein (p-STAT3/STAT3) expression in kidneys from the three groups. The numbers (1–13) indicate individual animals in a given group (n = 4–6). e, f Representative images and quantitative data showing the expression of p-STAT3 (brown) along with nuclei (blue) in each group to assess the effect of the EPRS1 inhibitor (n = 7–9). Scale bar = 50 μm. g, h Representative Western blot and quantitative data of protein (p-STAT3/STAT3) expression in the kidneys of the five groups. The numbers (1–13) indicate individual animals in a given group (n = 7–9). The data are presented as the means ± standard deviations. Statistical data were analyzed by ANOVA with Tukey’s post hoc test. *P < 0.05, **P < 0.01, and ***P < 0.001.
