Fig. 1: GRIM-19 levels are negatively correlated with those of STAT3 in the lesional skin of bleomycin-induced SSc mice. | Experimental & Molecular Medicine

Fig. 1: GRIM-19 levels are negatively correlated with those of STAT3 in the lesional skin of bleomycin-induced SSc mice.

From: GRIM-19-mediated induction of mitochondrial STAT3 alleviates systemic sclerosis by inhibiting fibrosis and Th2/Th17 cells

Fig. 1

Eight-week-old C57BL/6 mice were subcutaneously injected (n = 17) or not (normal control, n = 17) with 50 μg of bleomycin dissolved in 100 μL of PBS daily for 2 weeks. Tissues from SSc and control mice euthanized on Day 35 after the start of bleomycin treatment were subjected to histologic analysis. a Skin sections stained with hematoxylin and eosin (H&E) and Masson’s trichrome (MT). The thickness of the dermis and epidermis and the percentage of fibrosis area are plotted. Original magnification: 200×; scale bar: 100 µm. b Skin sections stained with antibodies against collagen type 1 (Col1) and α-smooth muscle actin (SMA). The number of antibody-positive cells (mean ± SEM) is plotted. Original magnification: 400×; scale bar: 100 µm. c Skin sections stained with antibodies against STAT3 and GRIM-19. The number of antibody-positive cells (mean ± SEM) is plotted. Original magnification: 400×; scale bar: 100 µm. d The correlations between α-SMA and STAT3, α-SMA and GRIM-19, and STAT3 and GRIM-19 were analyzed on the basis of numbers of α-SMA+, STAT3+, and GRIM-19+ cells in the skin of the SSc mice shown in (b and c). **p < 0.01, ***p < 0.001, ****p < 0.0001 (unpaired nonparametric t-test).

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