Fig. 4: PINK1 deletion-induced mitophagy impairment promotes the progression of bleomycin-induced SSc. | Experimental & Molecular Medicine

Fig. 4: PINK1 deletion-induced mitophagy impairment promotes the progression of bleomycin-induced SSc.

From: GRIM-19-mediated induction of mitochondrial STAT3 alleviates systemic sclerosis by inhibiting fibrosis and Th2/Th17 cells

Fig. 4

The SSc model was induced by subcutaneously injecting 50 μg of bleomycin dissolved in 100 μL of PBS daily for 2 weeks into 8-week-old mice lacking PINK1 and wild-type mice (n = 3/group). Tissues from SSc and control mice euthanized on Day 35 after the start of bleomycin treatment were subjected to histologic analysis. a Skin sections were stained with H&E and MT. The thickness of the dermis and epidermis and the percentage of tissue area involved in fibrosis are plotted. Original magnification: 100×; scale bar: 100 µm. b Skin sections stained with antibodies against Col1 and α-SMA. The number of antibody-positive cells (mean ± SEM) is plotted. Original magnification: 400×. Scale bar: 100 µm. **p < 0.01, ****p < 0.0001 (unpaired nonparametric t-test).

Back to article page