Fig. 1: PLCβ4 expression is upregulated during osteoclastogenesis.
From: Phospholipase C β4 promotes RANKL-dependent osteoclastogenesis by interacting with MKK3 and p38 MAPK
a, BMMs were cultured with 30 ng/ml M-CSF and 20 ng/ml RANKL for 4 days to induce osteoclast (OC) differentiation. b, Microarray profile of PLCβ4 expression during osteoclastogenesis. c, Quantitative real-time PCR was performed to assess the mRNA expression levels of the PLCβ isoforms (β1–β4) during osteoclastogenesis. d, PLCβ4 and PLCβ2 protein levels were determined by immunoblotting. e,f, BMMs were cultured with M-CSF and RANKL in the presence of the indicated inhibitors. PLCβ4 and PLCβ2 expression levels were analyzed via reverse-transcription PCR (e) or real-time PCR (f). BAY, Bay11-7802 (2 μM); SP, SP600125 (5 μM); PD, PD98059 (20 μM); SB, SB203580 (10 μM). Data are expressed as mean ± s.d. **P < 0.001 versus the vehicle.
