Fig. 3: Global deletion of PLCβ4 reduces osteoclastogenesis.
From: Phospholipase C β4 promotes RANKL-dependent osteoclastogenesis by interacting with MKK3 and p38 MAPK
a, Size comparison of 8-week-old WT and PLCβ4−/− mice. b, Alcian blue and alizarin red staining of the skeletons of WT and PLCβ4−/− mice at embryonic day 17.5. c, Deletion of PLCβ4 in PLCβ4−/− BMMs was confirmed by immunoblotting. The relative intensities of PLCβ4 and PLCβ2 are shown after normalization to β-actin. d,e, BMMs from WT and PLCβ4−/− mice were cultured with M-CSF (30 ng/ml) and RANKL (20 ng/ml) for 4 or 5 days: the cultured cells were fixed and subsequently subjected to TRAP staining (d); the number of osteoclasts was quantified (e). f, An MTS assay was performed, and the absorbance was measured at 490 nm. g, BMMs from WT and PLCβ4−/− mice were cultured in the presence of M-CSF and RANKL for the indicated days. The expression of osteoclast marker genes was measured by real-time PCR. All the data are expressed as mean ± s.d. *P < 0.05 and **P < 0.001 versus the WT.
