Fig. 4: Conditional deletion of PLCβ4 in the osteoclast lineage reduces osteoclastogenesis.
From: Phospholipase C β4 promotes RANKL-dependent osteoclastogenesis by interacting with MKK3 and p38 MAPK
a, Deletion of PLCβ4 in LysM-PLCβ4−/− BMMs and osteoclasts (OCs) was confirmed by immunoblotting. b, Comparison of the body weights of 8-week-old control and LysM-PLCβ4−/− male and female mice (n = 7–10). c,d, BMMs from control and LysM-PLCβ4−/− male and female mice were cultured with the indicated concentrations of RANKL in the presence of M-CSF (30 ng/ml): osteoclasts were visualized after TRAP staining (c): the number of osteoclasts was quantified (d). e, An MTS assay was performed, and the absorbance was measured at 490 nm. f,g, BMMs from control and LysM-PLCβ4−/− male and female mice were cultured in osteoclastogenic medium for the indicated days. The expression of the indicated genes was measured by real-time PCR (f) or immunoblotting (g). All the data are expressed as mean ± s.d. *P < 0.05 and #P < 0.001 versus the control; NS, not significant.
