Fig. 5: LysM-PLCβ4−/− male mice have increased bone mass.
From: Phospholipase C β4 promotes RANKL-dependent osteoclastogenesis by interacting with MKK3 and p38 MAPK
a, Representative µCT images of femurs from 8-week-old control and LysM-PLCβ4−/− male and female mice. b, Quantitative µCT analysis of trabecular bone parameters, including bone mineral density (BMD), bone volume per tissue volume (BV/TV), trabecular number (Tb.N), trabecular separation (Tb.Sp) and trabecular thickness (Tb.Th) (n = 7–10). c, Von Kossa staining of vertebral sections from 8-week-old control and LysM-PLCβ4−/− male mice. Scale bar, 1 mm. d, Histomorphometric quantification of the data from c (n = 7–8). e, TRAP-stained images of tibias from 8-week-old control and LysM-PLCβ4−/− mice. Scale bar, 200 μm. f, Quantification of the BV/TV, number of osteoclasts per bone surface (N.Oc/BS) and osteoclast surface per bone surface (Oc.S/BS) in e (n = 9). g, Calcein and alizarin red double-labeling images of trabecular bones in vertebrae from 8-week-old control and LysM-PLCβ4−/− mice. Scale bar, 20 μm. h, The mineral apposition rate (MAR) and bone formation rate (BFR/BS) were examined via histomorphometry of the images in g (n = 7–9). All the data are expressed as mean ± s.d. *P < 0.05; NS, not significant.
