Fig. 6: PLCβ4 modulates RANKL-mediated p38 phosphorylation.
From: Phospholipase C β4 promotes RANKL-dependent osteoclastogenesis by interacting with MKK3 and p38 MAPK
a,b, Serum-starved BMMs from control and LysM-PLCβ4−/− mice were stimulated with RANKL (50 ng/ml) for the indicated times. Immunoblotting was performed to assess IκBα phosphorylation and total IκBα (a) and the phosphorylation of p38, p65, JNK and ERK (b). c,d, Serum-starved BMMs from control and LysM-PLCβ4−/− mice were stimulated with M-CSF (50 ng/ml) (c) or TNF (10 ng/ml) (d) for the indicated times and analyzed by immunoblotting with antibodies specific for the indicated proteins.
