Fig. 1: The expression level of Acot12 in a CKD model.
From: ACOT12, a novel factor in the pathogenesis of kidney fibrosis, modulates ACBD5
a In silico analysis of Acot12 expression levels in GSE246576 (db/db mice), GSE150641 (adenine diet-fed mice; ADN, adenine diet-induced nephropathy) and GSE222570 (folic acid-injected mice; FAN, folic acid-induced nephropathy). b Gene Ontology enrichment analysis of differentially expressed genes in the GSE150641 dataset (adenine diet-induced CKD mice). c Immunohistochemical staining of ACOT12 in adenine diet-fed control (CL) and UUO kidneys. d Characteristics of patients who were diagnosed with chronic kidney obstruction. Immunohistochemical staining of ACOT12 in kidney fibrosis samples from patients. e In silico analysis of Acot12 expression levels in the GSE7869 dataset (human ADPKD, autosomal dominant polycystic kidney disease) and the correlation between Acot12 expression and the glomerular filtration rate calculated using the modification of diet in renal disease (MDRD) equation in the tubulointerstitium of ERCB lupus patients. The positive area was determined and is represented in a bar graph. Scale bar, 200 μm (n = 4). f Immunohistochemical staining and mRNA expression levels of ACOT12 in UUO kidneys (n = 6). The area positive for ACOT12 immunostaining was determined and is represented in a bar graph. Scale bar, 200 μm. The expression level of Acot12 in UUO mice was analyzed by RT‒PCR (n = 6). g Representative images of PLIN2 and ACOT12 immunostaining and Masson’s trichrome staining according to the intensity of ACOT12 staining. Pearson correlations between the positive areas of ACOT12 and PLIN2 staining or Masson’s trichrome staining are shown. **P < 0.01 and ***P < 0.001.
