Fig. 6: Restoration of Acot12 into Acot12−/−Pparα−/− kidneys reduces kidney fibrosis but restoration of PPARα does not. | Experimental & Molecular Medicine

Fig. 6: Restoration of Acot12 into Acot12−/−Pparα−/− kidneys reduces kidney fibrosis but restoration of PPARα does not.

From: ACOT12, a novel factor in the pathogenesis of kidney fibrosis, modulates ACBD5

Fig. 6

a Masson’s trichrome and immunohistochemical staining of PLIN2 and ACOT12 in Pparα−/− UUO kidneys with or without ACOT12 overexpression (ACOT12OE) (n = 3–5 per group). The positive area was determined and the data are presented as a bar graph. Scale bar, 200 μm. b The expression levels of fibrotic genes in Pparα−/− UUO kidneys with or without ACOT12 introduction were analyzed by RT‒PCR (n = 3‒5 per group). c Masson’s trichrome and immunohistochemical staining of αSMA, PLIN2, ACOT12 and PPARα in Acot12−/−Pparα−/− UUO kidneys without or with ACOT12 or PPARα overexpression (n = 4 per group). The positive area was determined and the data are presented as a bar graph. Scale bar, 200 μm. *P < 0.05, **P < 0.01 and ***P < 0.001 per sham or control CL. #P < 0.05 and ##P < 0.01 per control or ACOT12OE UUO.

Back to article page