Fig. 1: HSPCs are susceptible to SARS-CoV-2 PsV infection. | Experimental & Molecular Medicine

Fig. 1: HSPCs are susceptible to SARS-CoV-2 PsV infection.

From: SARS-CoV-2 pseudovirus dysregulates hematopoiesis and induces inflammaging of hematopoietic stem and progenitor cells

Fig. 1

a, Expression levels of the SARS-CoV-2 entry-related genes ACE2, DDP4 and TMPRSS2 in three different cell lines were determined by quantitative RT‒PCR (qRT‒PCR). b The protein level of ACE2 in MSCs, PBMCs and HSPCs was determined by western blotting. c Scheme of SARS-CoV-2 PsV infection. d SARS-CoV-2 PsV infection was confirmed through the detection of the viral spike sequence via qRT‒PCR. HSPCs were treated with the SARS-CoV-2 variants PsV, Wuhan, Delta and Omicron. e Differences between variants were determined through the relative expression levels of inflammatory genes. Gene expression of IL1β, IL6 and TNF in HSPCs infected with SARS-CoV-2 variants was determined by qRT‒PCR. f Flow cytometry analysis of GFP- and Omicron PsV-infected HSPCs. g The mean fluorescence intensity of ACE2 expression in GFP- or Omicron PsV-infected HSPCs was quantified using flow cytometry. Relative mRNA levels were normalized to those of GAPDH. *P < 0.05, **P < 0.01, ***P < 0.001. ns, not significant. The results are presented as the mean ± s.e.m.

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