Fig. 4: Hematopoietic reconstitution of NSG mice transplanted with Omicron PsV-infected HSPCs reveals inflammaging and myeloid bias. | Experimental & Molecular Medicine

Fig. 4: Hematopoietic reconstitution of NSG mice transplanted with Omicron PsV-infected HSPCs reveals inflammaging and myeloid bias.

From: SARS-CoV-2 pseudovirus dysregulates hematopoiesis and induces inflammaging of hematopoietic stem and progenitor cells

Fig. 4

a, Schematic representation of the in vivo experiment. This diagram illustrates the transplantation of Omicron PsV-infected HSPCs into NSG mice with and without NGO treatment (n = 6 per group). b Assessment of engraftment levels (hCD45+) via flow cytometry in mouse PB 12 weeks after transplantation. c, d Percentages of hCD34+ cells (c) and LT-HSCs (d) in mouse BM were determined by flow cytometry. e Transcription levels of inflammaging markers in mouse BM were determined by qRT‒PCR. Relative mRNA levels were normalized to those of GAPDH. f RNA sequencing was performed to evaluate the phenotype of BM human cells in mice transplanted with Omicron PsV-infected HSPCs. KEGG pathway analysis and GO enrichment analysis of DEGs. g IPA analysis of BM cells in mice transplanted with Omicron PsV-infected HSPCs. *P < 0.05, **P < 0.01, ***P < 0.001. The results are shown as the mean ± s.e.m.

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