Fig. 2: LncRNA Gm35585 reduces HFD-induced hepatocytic lipid accumulation in mice.
From: LncRNA Gm35585 transcriptionally activates the peroxidase EHHADH against diet-induced fatty liver
a In vivo experimental flow diagram. NCD mice: C57BL/6J mice; HFD mice: 10 weeks of high-fat feeding C57BL/6J mice. b Changes in the body weight of mice within 10 days after AAV-Gm35585 injection (mean ± s.d., n = 5, one-way ANOVA; ****P < 0.0001, NCD + AAV-NC versus HFD + AAV-NC; #P < 0.05, ##P < 0.01, ###P < 0.001, HFD + AAV-NC versus HFD + AAV-Gm35585). c Changes in liver TG and TC levels under Gm35585 overexpression condition (mean ± s.d., n = 5; one-way ANOVA). d Serum biochemical indices included ALT, AST, TG, TC, LDL, and HDL (mean ± s.d., n = 5; one-way ANOVA). e Oil Red O staining of liver tissue sections indicating changes in lipid droplet content. Scale bar, 50 μm. f Differences between groups after Gm35585 overexpression (n = 3). g The nnumber of upregulated and downregulated genes after Gm35585 overexpression (n = 3). h KEGG enrichment analysis of RNA sequencing data from HFD + AVV-Gm35585 mouse livers.
