Fig. 3: Gm35585 ameliorates hepatic lipid accumulation by regulating EHHADH.
From: LncRNA Gm35585 transcriptionally activates the peroxidase EHHADH against diet-induced fatty liver
a The gene expression of Pparα, Acc, Fas, Srebp1, Fatp1, Cd36, Ehhadh, Acox1, Apob and Mttp in vivo. The values were normalized to those of the control group (mean ± s.d., n = 5; two-way ANOVA). b The protein expression of PPARα, EHHADH and CD36 in vivo. c The gene expression of Pparα, Acc, Fas, Srebp1, Fatp1, Cd36, Ehhadh, Acox1, Apob and Mttp in AML-12 cells. The values were normalized to those of the control group (mean ± s.d., n = 3, two-way ANOVA). d The protein expression of PPARα and EHHADH in AML-12 cells. e Costaining of liver tissue sections with Nile Red and EHHADH immunofluorescence in AML-12 cells. Scale bar, 25 μm. f Costaining of liver tissue sections with Nile Red and EHHADH immunofluorescence in HepG2 cells. Scale bar, 50 μm. g Changes in the body weight of mice within 1 month after TBG-EHHADH injection. NCD mice: C57BL/6J mice; HFD mice: 5 weeks of high-fat feeding of C57BL/6J mice. h Serum biochemical indices included ALT, AST, TG, TC, LDL-C and HDL-C (mean ± s.d., n = 5; one-way ANOVA). i Oil Red O staining of liver tissue sections indicating changes in lipid droplet content. Scale bar, 50 μm. j Changes in liver TG and TC levels (mean ± s.d., n = 5; one-way ANOVA). k Changes in the serum FFA levels (mean ± s.d., n = 5; one-way ANOVA). l Changes in the liver FFA levels (mean ± s.d., n = 5; one-way ANOVA).
