Fig. 4: Gm35585 promotes oxidation of LCFAs contents by activating peroxidase EHHADH.
From: LncRNA Gm35585 transcriptionally activates the peroxidase EHHADH against diet-induced fatty liver
a, f Costaining of liver tissue sections with Nile Red and EHHADH immunofluorescence. Scale bar, 25 μm. b, g Liver peroxidase activity. Reaction time: 30 min (mean ± s.d., n = 5, one-way ANOVA). c, h Liver total FA contents analyzed by LC–MS/MS (mean ± s.d., n = 5, one-way ANOVA). d, i Heat map of 28 FA contents in the liver. *P < 0.05, **P < 0.01, ***P < 0.001, NCD+AAV-NC vs HFD+AAV-NC, and NCD+TBG-NC vs HFD+TBG-NC. #P < 0.05, ##P < 0.01, ###P < 0.001, HFD+AAV-NC vs HFD+AAV-Gm35585, and HFD+TBG-NC vs HFD+TBG-EHHADH. e, j Changes in hepatic SCFA, MCFA and LCFA levels. Information on the 28 FAs is given in Table 4. (a–e The analysis of Gm35585 overexpression experiments in vivo. f–j The analysis of EHHADH overexpression experiments in vivo).
