Fig. 6: SGI-1776 inhibits LPS-induced bone resorption in vivo.
From: Pim1 promotes the maintenance of bone homeostasis by regulating osteoclast function
a Schematic depiction of the experiment. Murine calvariae were injected twice with PBS (n = 9), LPS alone (12.5 mg/kg body weight alone (n = 9)), or LPS combined with either 2.5 mg/kg (n = 9) or 10 mg/kg body weight (n = 10) SGI-1776. b, c After euthanasia, the calvariae were sectioned and subjected to TRAP staining and histomorphometry: representative images of the TRAP-stained calvariae (scale bars, 100 μm) (b); the bone erosion was quantified and expressed as the percentage of eroded surface relative to total bone surface (ES/BS) (c). d Histomorphometry to determine the No.OCs. The results are presented as mean ± s.d. Statistical differences were analyzed by using one-way ANOVA with Tukey’s multiple comparisons test. ns, not significant. ****P < 0.0001.
