Fig. 2: Identification of signaling pathways, TFs and their target genes associated with H3K23su DEGs.

a, b Top 10 KEGG pathway-associated DEGs related to the H3K23su GAIN (a) and LOSS (b) regions. DEGs are shown in a color gradient based on their log₂fold change (FC) values. c The top 25 enriched known TF motifs of the H3K23su GAIN regions, with P values estimated from HOMER (v.4.10). The AP-1, FOX and GATA family members are colored blue, red and green, respectively. The percentages of target sequences of DERs with TF motifs are indicated by polygonal chains in black. d Venn diagram showing the overlap between the corresponding TFs for each motif identified by HOMER (v.4.10) in the H3K23su GAIN regions and the upregulated DEGs identified by RNA-seq. The dot plot (right) shows the identified upregulated TFs. The color of each dot represents the P value of TF enrichment, and only TFs with P values < 0.01 were included. The size of each dot represents the expression level of the corresponding TF, log₂(FPKM + 1). FPKM, Fragments Per Kilobase of exon model per Million mapped fragments. e Predicted TFBSs by HOMER analysis of representative DEGs positively correlated with H3K23su changes. The dark-blue arrows indicate the TSS and the direction of gene transcription. f, g Top: line charts showing the distribution probability of representative FOSL2-binding motifs around CUT&Tag peak summits in H3K23su DERs. Bottom: histograms indicating the expression levels of predicted TF (FOSL2) target DEGs in H3K23su DERs identified by HOMER (v.4.10). Only DEGs with TFBSs in the promoter-TSS regions were included. DEGs are listed in descending order based on the log₂fold change.