Fig. 6: KLF6 recruited the p300 complex to enhance the succinylation of H3K23. | Experimental & Molecular Medicine

Fig. 6: KLF6 recruited the p300 complex to enhance the succinylation of H3K23.

From: KLF6-mediated recruitment of the p300 complex enhances H3K23su and cooperatively upregulates SEMA3C with FOSL2 to drive 5-FU resistance in colon cancer cells

Fig. 6

a IP-MS analysis revealed that TAF6L was at the top of the list. b KLF6 interacted with TAF6L, as shown by co-IP analysis. c Colocalization of KLF6 and PCAF in the nucleus of HCT15-FR cells by IF. d Colocalization of KLF6 and p300 in the nucleus of HCT15-FR cells by IF. e KLF6 interacted with PCAF, as shown by co-IP analysis. f KLF6 interacted with p300, as shown by co-IP analysis. g Western blotting revealed that H3K23su decreased as PCAF was knocked down. h Western blotting revealed that H3K23su decreased as p300 was knocked down. i H3K23su, PCAF and p300 decreased in the nucleus of HCT15-FR cells with KLF6 knockdown, whereas PCAF and p300 remained unchanged in the cytosol.

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