Fig. 7: FOSL2 overexpression or knockdown induced an increase or decrease in 5-FU resistance, which could be reversed by KLF6 knockdown or overexpression.

a FOSL2 knockdown in HCT15-FR cells downregulated the 5-FU-resistance-promoting proteins SEMA3C, β-catenin, TCF1 and c-Myc. When KLF6 was overexpressed, this downregulation was impaired. b The decrease in 5-FU resistance induced by FOSL2 knockdown in HCT15-FR cells could be rescued by KLF6 overexpression through colony formation assays. c The IC₅₀ values suggested that KLF6 overexpression could reverse the FOSL2 knockdown-induced decrease in 5-FU resistance in HCT15-FR cells. d A nude mouse subcutaneous tumor model indicated that KLF6 overexpression could rescue the decrease in 5-FU resistance induced by FOSL2 knockdown. The tumor volume was measured every 3 days after injection. The tumor weights of the subcutaneous xenografts are shown. e SEMA3C, β-catenin, TCF1 and c-Myc, which induce an increase in 5-FU resistance in HCT15-FR cells, were downregulated by si-p300. This downregulation was reversed by KLF6 overexpression. f Colony formation assays indicated that KLF6 overexpression reversed the p300 knockdown-induced decrease in 5-FU resistance in HCT15-FR cells. g The IC₅₀ values suggested that KLF6 overexpression reversed the p300 knockdown-induced decrease in 5-FU resistance in HCT15-FR cells. h A nude mouse subcutaneous tumor model indicated that KLF6 overexpression rescued the decrease in 5-FU resistance induced by p300 knockdown. The tumor volume was measured every 4 days after injection. The tumor weights of the subcutaneous xenografts are shown.