Fig. 1: Toxic effects of Sev on hNPCs/neurons in vitro. | Experimental & Molecular Medicine

Fig. 1: Toxic effects of Sev on hNPCs/neurons in vitro.

From: Human neuron chimeric mice reveal impairment of DVL-1-mediated neuronal migration by sevoflurane and potential treatment by rTMS

Fig. 1

a The experimental design for b–g. b, c Immunostaining (b) and western blotting (c) of Tuj-1 in hNPCs treated with or without Sev. d, e Immunostaining (d) and western blotting (e) of VGLUT1 in human neurons treated with or without Sev. f, g Immunostaining (f) and western blotting (g) of PSD-95 in human neurons treated with or without Sev. Notice the reduction of Tuj-1, VGLUT1 and PSD-95 following Sev treatment. h, i Representative images of migrating hNPCs from neurospheres treated with or without Sev (h) and the quantification of migrating cells and migrating distance (i). j, k Double immunostaining of Tuj-1/Sox2 in hCOs and the quantification of Tuj-1+ cells in the CP. Notice the suppression of human neuronal migration in Sev-treated neural spheres and hCOs. N = 3 batches of cells per group in b–g and 4–5 batches of cells per group in h–k. Student’s t-test. Error bars show s.e.m. *P < 0.05, **P < 0.01 and ***P < 0.001. Con control, IF immunofluorescence, WB western blot.

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