Fig. 4: Downregulation of DVL-1/Ca2+ noncanonical Wnt signaling by Sev treatment. | Experimental & Molecular Medicine

Fig. 4: Downregulation of DVL-1/Ca2+ noncanonical Wnt signaling by Sev treatment.

From: Human neuron chimeric mice reveal impairment of DVL-1-mediated neuronal migration by sevoflurane and potential treatment by rTMS

Fig. 4

a A heat map of gene expression profiles of control and Sev-treated human neurons. b, c GO enrichment of the top 20 significantly changed biological processes (b) and qPCR verification of genes related to migration (c). d The top ten significantly changed signaling pathways. e, f Western blot validation of noncanonical Wnt signaling components in control and Sev-treated human neurons. g, h The calcium response of control and Sev-treated human neurons to KCl. Notice the significant downregulation of noncanonical Wnt signaling molecules and reduced calcium response in Sev-treated human neurons. N = 3 batches of cells. *P < 0.05 and **P < 0.01. One-way ANOVA in c and f and Student’s t-test in h (right). The error bars show s.e.m. Con control, DVL-1 disheveled-1, FGF17 fibroblast growth factor 17, LHX1 LIM homeobox protein 1, MIXL1 mix paired-like homeobox, PITX2 paired-like homeodomain 2, STC1 stanniocalcin 1, TEK TEK receptor tyrosine kinase.

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