Fig. 6: Effects of rTMS on cell migration and DVL-1/Ca2+ signaling of Sev-pretreated hNPCs in vitro. | Experimental & Molecular Medicine

Fig. 6: Effects of rTMS on cell migration and DVL-1/Ca2+ signaling of Sev-pretreated hNPCs in vitro.

From: Human neuron chimeric mice reveal impairment of DVL-1-mediated neuronal migration by sevoflurane and potential treatment by rTMS

Fig. 6

a The experimental design for b–h. b–d Ca2+ imaging of hNPCs treated with Sev or Sev plus rTMS, and the quantification. e, f Western blotting of Wnt5a, DVL-1, CaMKII and Rho-A in hNPCs treated with Sev or Sev plus rTMS (e), and the quantification (f). g, h Phase-contrast imaging (g) and quantification (h) of cell migration in hNPCs treated with Sev or Sev plus rTMS. N = 3 batches of cells. Student’s t-test in d and h and one-way ANOVA in f. *P < 0.05, **P < 0.01 and ***P < 0.001. Con control, WB western blot.

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