Fig. 7: Improvement of human neuronal migration and social function in Sev-pretreated chimeric mice by precisely targeted rTMS.
a The experimental scheme for e–l. b, c Stimulation of the left M1 region by precisely targeting rTMS (b) and the corresponding electromyographic activity (c). d Double immunostaining of c-Fos/GFP in an hNPC graft after rTMS treatment. Notice the c-Fos expression in the hNPC graft but not in mouse brain tissue. e Double immunostaining of c-Fos/GFP in an hNPC graft of Sev-pretreated chimeric mice (preSev) that had been treated with rTMS or not, and quantification. f Double immunostaining of DCX/GFP in Sev-pretreated chimeric mice with or without rTMS treatment. rTMS treatment increased the migration of DCX+ cells. g Western blotting of DVL-1, CaMKII, Rho-A and Rac-1 in Sev-pretreated chimeric mice with or without rTMS treatment. h–j A three-chamber assay on Sev-pretreated chimeric mice with or without rTMS treatment. k–l A resident–intruder assay on Sev-pretreated chimeric mice with or without rTMS treatment. Notice the improvement of social preference and social interaction by rTMS treatment. N = 3–4 mice per group in c–g and 8–10 mice per group in f–l. Student’s t-test in i (left), j and l and one-way ANOVA in g and i (right). *P < 0.05, **P < 0.01 and ***P < 0.001. Con control.
