Fig. 4: LpEV treatment reversed the altered expression of genes for vascular development and nucleotide metabolic process in the NAc of Drd2-KO mice. | Experimental & Molecular Medicine

Fig. 4: LpEV treatment reversed the altered expression of genes for vascular development and nucleotide metabolic process in the NAc of Drd2-KO mice.

From: Lactobacillus paracasei-derived extracellular vesicles reverse molecular and behavioral deficits in mouse models of autism spectrum disorder

Fig. 4

a–e, Volcano plots of NAc RNA seq datasets (a). RRHO map depicting possible gene expression patterns changed by LpEV in Drd2-KO mice (b). A RRHO map with gene expression in the NAc of Drd2-KO mice (WT versus KO) and gene expression changed by LpEV in Drd2-KO mice (KO versus KO + LpEV) (c). Genes exclusively located within quadrants B and C were selected for further analysis. d,e, Serial k-means clustering and GO enrichment analysis of genes in quadrant B (666 genes) (d) and quadrant C (544 genes) (e) grouped into functional clusters with distinct BPs. We selected k = 5. f–i, Two functional clusters of genes in quadrant B. Cluster 1 (195 genes) contained genes involved in ‘vascular development and tyrosine kinase signaling’ (f). Cluster 2 (99 genes) contained genes regulating ‘nucleotide metabolic process’ (h). The interaction networks of genes within the selected BPs (red; g and i). Clusters 3, 4 and 5 in quadrant B are provided in Supplementary Fig. 5a–f. j–m, Two functional clusters of genes in quadrant C. Cluster 1 (163 genes) harbored genes involved in ‘cell cycle and chromosome organization’ (j). Cluster 2 (138 genes) contained genes for ‘positive regulation of transcription, DNA-templated’ (l). The interaction networks of genes within the selected BPs are in blue (k and m). Clusters 3 and 4 in quadrant C are provided in Supplementary Fig. 5g–i.

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