Table 1 Sequences for quantitative PCR with reverse transcription primers or siRNA targets.
Gene names | Sequences | |
|---|---|---|
Forward primer (5′ → 3′) | Reverse primer (5′ → 3′) | |
ITGA4 | TGAATGTGTCCTTGTTTAATGCTG | TTGTACCACGCCAGAGTTATC |
ITGA5 | ATACTCTGTGGCTGTTGGTG | CTGTTCCCCTGAGAAGTTGTAG |
DMD (Dystrophin) | GAACTGGCTGCTGAATGTTTATG | CGCTTCGATCTCTGGCTTATT |
SLC1A5 | GAAGTGCGTGGAGGAGAATAA | CAGCCAGGATCAAGGAGATATG |
GAPDH | CCAGCCGAGCCACATCGCTC | ATGAGCCCCAGCCTTCTCCAT |
Gene names | siRNA target sequence | |
Forward (5′ → 3′) | Reverse (5′ → 3′) | |
ITGA2 | GAAACGCCCUUGAUACUAAAAAUTT | AAAUUUUUAGUAUCAAGGGCGUUUCUG |
NCOA3 | AAACCAGCAGAAUAUCAUGAUUUCT | AGAAAUCAUGAUAUUCUGCUGGUUUGG |
ITGA4 | GGUACUGCAUUCUGAAUCAGAAUCT | AGAUUCUGAUUCAGAAUGCAGUACCUG |
ITGA5 | CUACAAGCUUGGAUUCUUCAAACGC | GCGUUUGAAGAAUCCAAGCU UGUAGAG |
DMD (Dystrophin) | CUUAGUAUCAGUCAUGACAGAUGAA | UUCAUCUGUCAUGACUGAUACUAAGGA |
SLC1A5 | GAUCUUGCGAGAAAUAUCUUCCCTT | AAGGGAAGAUAUUUCUCGCAAGAUCC |
siTM4SF5#4 | CCA UCU CAG CUU GCA AGU C | GAC UUG CAA GCU GAG AUG G |
siTM4SF5#7 | CCUCCU GCU GGU ACC UAA U | AUU AGG UAC CAG CAG GAG G |
siNS | GenePharma negative control no. A06001 | |
