Fig. 4: The genomic alterations in iPS cells and iMS cells through reprogramming, differentiation and passaging. | Experimental & Molecular Medicine

Fig. 4: The genomic alterations in iPS cells and iMS cells through reprogramming, differentiation and passaging.

From: Tracing genomic instability in induced mesenchymal stromal cell manufacture: an integration-free transfection approach

Fig. 4

a, Nine iPS cells were produced using two integration-free methods: SV (top, SV-iPS cell) and episomal vectors (bottom, Epi-iPS cell). Two iMS cell lines differentiated from each iPS cell lines are depicted, including their further passages. In vivo genomic stability was assessed using teratomas, symbolized by a mouse. b, Reprogramming-induced CNAs were found in all SV-iPS cells and two Epi-iPS cells. All differentiated iMS cell lines were found to carry exhibited CNA or SNVs. Notably, the all detected genomic aberrations were distinct and showed no recurring pattern. Each alteration was validated using independent methodologies.

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