Fig. 1: Multi-omics analysis suggests radiotherapy promotes cholesterol synthesis in tumor tissue. | Experimental & Molecular Medicine

Fig. 1: Multi-omics analysis suggests radiotherapy promotes cholesterol synthesis in tumor tissue.

From: Cholesterol biosynthesis induced by radiotherapy inhibits cGAS–STING activation and contributes to colorectal cancer treatment resistance

Fig. 1

a Plot of the orthogonal projections to latent structures discriminant analysis (OPLS-DA) score for metabolomics in the two groups. b Volcano plots of the metabolomics with significant changes after radiotherapy. c Bar chart of metabolite sets enrichment analysis (MSEA) of differential metabolites in the two groups. d The top 20 significantly enriched pathways in GO terms for biological process (BP). e Heatmap of log2 fold change (FC) to depict the gene expression associated with cholesterol biosynthesis. f GSEA of cholesterol biosynthetic process (GO: 0006695) between the two groups. g The cholesterol levels in CT26 tumor tissues on the 10th day after local 6 Gy irradiation. h The cholesterol levels in CT26 and HCT116 cells at 24 h after exposure to 0, 2, 4 and 6 Gy irradiation. i The cholesterol levels in CT26 and HCT116 cells at different times (0, 12, 24, 48 and 72 h) after exposure to 6 Gy irradiation. j, k At 24 h after exposure to 0, 2, 4 and 6 Gy, CT26 (j) and HCT116 cells (k) were labeled with Hoechst (blue) and cholesterol-BODIPY (green). Scale bar, 10 μm. **P < 0.01; *P < 0.05; ns, not significant.

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