Fig. 2: Radiation exposure precipitates cholesterol synthesis by upregulating HMGCR expression in CT26 tumors and CRC cells. | Experimental & Molecular Medicine

Fig. 2: Radiation exposure precipitates cholesterol synthesis by upregulating HMGCR expression in CT26 tumors and CRC cells.

From: Cholesterol biosynthesis induced by radiotherapy inhibits cGAS–STING activation and contributes to colorectal cancer treatment resistance

Fig. 2

a The relative expression of HMGCR, SREBP2, SQLE and AMPK mRNA extracted from CT26 tumors was detected by qPCR. b The expression of HMGCR protein extracted from CT26 tumors was detected by western blot. c The relative expression of HMGCR mRNA extracted from CT26 and HCT116 cells at 24 h after exposure to different doses of irradiation (0, 2, 4 and 6 Gy) was detected by qPCR. d The relative expression of HMGCR mRNA extracted from CT26 and HCT116 cells at different times (0, 12, 24, 48 and 72 h) after exposure to 6 Gy irradiation was detected by qPCR. e The expression of HMGCR protein extracted from CT26 and HCT116 cells at 24 h after exposure to different doses of irradiation (0, 2, 4 and 6 Gy) was detected by western blot. f The expression of HMGCR protein extracted from CT26 and HCT116 cells at different times (0, 12, 24, 48 and 72 h) after exposure to 6 Gy irradiation was detected by western blot. g The cholesterol levels in CT26 and HCT116 cells with siHMGCR interference at 24 h after exposure to 6 Gy irradiation. **P < 0.01; *P < 0.05; ns, not significant.

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