Fig. 7: HMGCR inhibition combined with radiotherapy significantly activates the cGAS–STING pathway. | Experimental & Molecular Medicine

Fig. 7: HMGCR inhibition combined with radiotherapy significantly activates the cGAS–STING pathway.

From: Cholesterol biosynthesis induced by radiotherapy inhibits cGAS–STING activation and contributes to colorectal cancer treatment resistance

Fig. 7

a, A volcano plot of differentially expressed genes between the combination group and the radiotherapy group. b GSEA of the TCR signaling pathway (KEGG: MMU04660) and T-cell-mediated immunity (GO: 0002456) between the combination therapy group and the radiotherapy group. c A heatmap of log2FC to depict the gene expression associated with type I IFN. d The expression of p-TBK1, p-IRF3, TBK1 and IRF3 protein extracted from CT26 tumors was detected by western blot. e, The relative expression of CCL5, CXCL10 and IFNβ mRNA extracted from CT26 tumors was detected by qPCR. f, Representative IF images of p-TBK1 and p-IRF3. g, Representative IHC images of IFN-β. h, The levels of IFN-β, CCL5 and IFN-γ protein in tumor tissues were measured via ELISA. Scale bar, 50 μm. **P < 0.01; *P < 0.05.

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