Fig. 1: Mitochondrial function and morphological characterization in somatic cells and PSCs.
From: Changes in mitochondrial thymidine metabolism and mtDNA copy number during induced pluripotency
a Phase-contrast microscopic (PCM) images depicting fibroblasts (MRC5; scale bar, 50 μm) and PSCs (hiPSC, H9-hESC; scale bar, 100 μm). Transmission electron microscopic (TEM) images of fibroblasts (MRC5) and PSCs (hiPSC, H9-hESC). Scale bar, 1 μm. Red arrows indicate mitochondria. b Mito stress test conducted on somatic cells and PSCs. Mito stress test data profiles for OCR and calculated values for respiratory parameters. Results are presented as means ± s.d. (n = 3). c Assessment of reliance on respiration or glycolysis in fibroblasts and PSCs as illustrated by the plotted OCR/ECAR ratios. Results are presented as means ± s.d. (n = 6). d mtDNA-to-nDNA ratios in fibroblasts and PSCs. Results are presented as means ± s.d. (n = 3). *P < 0.05, **P < 0.005, ***P < 0.0005, ****P < 0.00005). n.s., nonsignificant. Significant differences were assessed using Student’s t-test or one-way ANOVA with Tukey’s multiple-comparison test. Statistical analyses were performed using GRAPHPAD 8.0.1.ESC embryonic stem cell, PSC pluripotent stem cell.
