Fig. 3: Inhibitory effect of TK2 in fibroblasts.
From: Changes in mitochondrial thymidine metabolism and mtDNA copy number during induced pluripotency
a Western blot analysis of TK2 expression in fibroblasts (BJ1) after 72 h of treatment with AZT (a TK2 inhibitor). b Alterations in the mtDNA/nDNA ratio in fibroblasts (BJ1) after AZT treatment. Results are expressed as means ± s.d. (n = 3). c Changes in mitochondrial OXPHOS complex expression in fibroblasts (BJ1) after AZT treatment. d Real-time measurement of the oxygen consumption rate (OCR) during mitochondrial stress test in BJ1 fibroblasts treated with 50 μM AZT for 72 h. OCR was monitored at baseline and following sequential addition of oligomycin, CCCP, and a mixture of antimycin A and rotenone. e Quantitative analysis of mitochondrial respiration parameters derived from Seahorse assay shown in (d), including basal respiration, maximal respiration, proton leak, ATP production, and spare respiratory capacity. Results are presented as means ± s.d. (n = 4). Statistical significance was determined by Student’s t-test (*P < 0.05) d, e, Modulations in Mito stress test profiles and respiratory parameters in fibroblasts (BJ1) after 72 h of treatment with 50 μM AZT. Results are presented as means ± s.d. (n = 4). *P < 0.05. Significant differences were analyzed using Student’s t-test. Statistical analyses were conducted using GRAPHPAD 8.0.1. ESC, human embryonic stem cell, PSC pluripotent stem cell.
