Fig. 5: Assessment of somatic cell reprogramming efficiency using an SIRT1 inhibitor (sirtinol) and activator (SRT1720).
From: Changes in mitochondrial thymidine metabolism and mtDNA copy number during induced pluripotency
a, Flow cytometric analysis of the surface markers, TRA-1-60 (FITC) and SSEA4 (APC), in sirtinol- and SRT1720-treated reprogrammed cells. b, Statistical analysis of somatic cell reprogramming efficiency based on FACS data. c, Alterations in the protein expression patterns of SIRT1, p53 acetylation, p21, TK2 and NANOG due to SIRT1 regulation during somatic cell reprogramming. d, Changes in the mtDNA/nDNA ratio caused by SIRT1 regulation during somatic cell reprogramming. Results are presented as means ± s.d. (n = 3). The asterisk denotes statistical significance. Significant differences were assessed using Student’s t-test or one-way ANOVA and Tukey’s multiple-comparison test. Statistical analyses were conducted using GRAPHPAD 8.0.1.
