Fig. 2: Elevated RGC apoptosis in experimental myopia. | Experimental & Molecular Medicine

Fig. 2: Elevated RGC apoptosis in experimental myopia.

From: Single-cell RNA sequencing reveals anterograde trans-synaptic degeneration and exacerbated synaptic remodeling in myopia

Fig. 2

a FOS staining of the retina after myopic induction for 4 weeks (4w) and 6 weeks (6w). Green fluorescence represents apoptotic cells. b Bax and Bcl-2 staining of the retina after myopic induction for 4 and 6 weeks. Red fluorescence represents apoptotic cells. c TUNEL staining of the retina after myopic induction for 4 weeks. Green fluorescence represents apoptotic cells. d Retinal Nissl staining. Red arrows indicate RGCs and neuron particles are in dark blue (n = 3). e, f The 5 min data of Ca2+ in the retina recorded by NMT after myopic induction for 4 (e) and 6 (f) weeks (n = 4). g Bar graphs of FOS levels after myopic induction for 4 and 6 weeks (***P < 0.001) (n = 3). h, i Bar graphs of Bax (h) and Bcl-2 (i) levels after myopic induction for 4 and 6 weeks (n = 3). j Bar graphs of apoptosis percentage according to the TUNEL analysis after myopic induction for 4 weeks (n = 3). k Bar graphs of Nissl staining results of RGCs at 4 and 6 weeks after myopia induction (**P < 0.01) (n = 3). l Analysis of the retinal Ca2+ based on NMT for 4 weeks (***P < 0.001) (n = 4).

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