Fig. 1: PRMT7 inhibition aggravates stress-induced ER stress and enhances cell death in C166 cells. | Experimental & Molecular Medicine

Fig. 1: PRMT7 inhibition aggravates stress-induced ER stress and enhances cell death in C166 cells.

From: Endothelial PRMT7 prevents dysfunction, promotes revascularization and enhances cardiac recovery post-myocardial infarction

Fig. 1

a qRT-PCR analysis of Prmt7 mRNA expression in C166 cells treated with VEGFA (100 ng/ml), TNF-α (50 ng/ml) and TN (10 µg/ml) for different time durations (0, 6, 12 and 24 h). b qRT-PCR analysis of Bax, p53 and Bnip3 mRNA expression in C166 cells treated with TNF-α (50 ng/ml) for different time durations (0, 6, 12 and 24 h). c qRT-PCR analysis of Bcl2, Mki67, p53 and Bax mRNA expression in C166 cells treated with dimethyl sulfoxide (DMSO), SGC8158 (1 µM), TNF-α (50 ng/ml) or a combination of SGC8158 and TNF-α for 24 h. d Representative images of BrdU staining (red) and DAPI (blue) in C166 cells treated under the same conditions as in c. Scale bar, 50 µm. e Quantification of the percentage of BrdU-positive cells. f Representative images of the scratch wound healing assay in C166 cells treated under the same conditions as in c for 0 h or 24 h. Scale bar, 100 µm. Quantification of wound closure percentage from the scratch wound healing assay. g Representative images of the tube formation assay in C166 cells treated under the same conditions as in c. Scale bar, 50 µm. Quantification of tube number. h Representative images of immunostaining for DNA damage (γH2AX, red) and counterstained with DAPI (blue) in C166 cells treated under the same conditions as in c. Scale bar, 10 µm. Quantification of average intensity of γH2AX in C166 cells. i qRT-PCR analysis of Atf4 and Chop mRNA expression in C166 cells. j Immunoblot analysis of HSP70, p-eIF2α, eIF2α, CHOP, ATF4 and loading control (GAPDH) protein levels in C166 cells treated with DMSO, SGC8158 (1 µM), TN (10 µg/ml) or a combination of SGC8158 and TN for 24 h. k Representative images of immunostaining for CHOP (red), and counterstained with DAPI (blue), in C166 cells treated with DMSO, SGC8158 (1 µM), TN (10 µg/ml) or a combination of SGC8158 and TN for 24 h. Scale bar, 10 µm. Quantification of the average intensity of CHOP. All data are presented as mean ± s.d. One-way ANOVA. ns, P > 0.05; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

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