Fig. 2: Endothelial PRMT7 ablation exacerbates MI-induced cardiac dysfunction. | Experimental & Molecular Medicine

Fig. 2: Endothelial PRMT7 ablation exacerbates MI-induced cardiac dysfunction.

From: Endothelial PRMT7 prevents dysfunction, promotes revascularization and enhances cardiac recovery post-myocardial infarction

Fig. 2

a Electrocardiography parameters of sham, MI and endothelial PRMT7-knockout (EndoKO)-MI mice 1 and 3 weeks after MI. b Representative echocardiographic images of sham (S), MI (M) and EndoKO-MI (EM) mice 3 weeks after MI. c EF and FS of S, MI and EM 3 weeks after MI. d LV mass 3 weeks after MI. e Representative images of hearts stained with Evans blue 3 weeks after MI. f Relative heart weight normalized by the body weight 3 weeks after MI. g H&E staining and Masson’s trichrome staining of hearts 3 weeks after MI. Scale bar, 50 µm. h Quantification of necrosis and fibrosis area of heart tissues. i Representative images of TUNEL (red), staining with cardiomyocyte marker (TNNT2, green) and counterstained with DAPI (blue). Scale bar, 20 µm. Quantification of the percentage of TUNEL-positive cells. j Immunoblot analysis of collagen type I (COL-1), p53, BAX and loading control (GAPDH) protein levels in heart lysates from S, M and EM mice. Quantification of COL-1, p53 and BAX levels relative to GAPDH (n = 3). All data are presented as mean ± s.d. One-way ANOVA. ns, P > 0.05; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

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