Fig. 3: PRMT7 ablation in ECs alters angiogenesis, cell proliferation and apoptosis-related gene expression after MI.

a Representative GO terms of enriched biological process on the upregulated genes from murine MI samples (GSE201947). b Representative images of heart sections stained for CDH5 (green) and TNNT2 (red) and counterstained with DAPI (blue). Scale bar, 20 µm. Quantification of capillary number in the heart tissues of sham (S), MI (M) or EndoKO-MI (EM) mice. c Representative images of heart sections stained for CDH5 (green) and MKI67 (red) and counterstained with DAPI (blue). Scale bar, 20 µm. Quantification of the percentage of MKI67⁺ CDH5⁺ cells in the heart tissues. d Immunoblot analysis of VEGFR2, CDH5 and GAPDH protein levels in heart lysates. e Quantification of VEGFR2 and CDH5 protein levels relative to GAPDH (n = 3). f Representative images of heart sections stained for CDH5 (green) and γH2AX (red) and counterstained with DAPI. Scale bar, 20 µm. Quantification of the percentage of γH2AX⁺CDH5⁺ cells in the heart tissues. g Representative images of heart sections stained for TNNT2 (green) and γH2AX (red) and counterstained with DAPI (blue). Scale bar, 20 µm. Quantification of the percentage of γH2AX⁺ cardiomyocytes in the heart tissues. h Immunoblot analysis of PRMT7, CHOP, ATF4, p-eIF2α, eIF2α, γH2AX and GAPDH protein levels in heart lysates. i Quantification of p-eIF2α relative to eIF2α, PRMT7, CHOP, ATF4 and γH2AX relative to GAPDH (n = 3). All data are presented as mean ± s.d. One-way ANOVA. ns, P > 0.05; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.