Fig. 1: Regional transcriptome analysis in the human hippocampus identifies dentate gyrus-specific FGFR1 elevation in MDD.

a A schematic representation of patients with MDD and control human postmortem hippocampus performing the regional dissection and analysis. b Heat maps showing expression levels of the top 200 genes in each subregion of the hippocampus–dentate gyrus, CA1, CA2, CA3, with enriched GO terms. c A heat map showing the expression levels of filtered genes observed in DAVID analysis. d A schematic table showing filtering process of expression levels of the genes in each region with hippocampal subregions of patients with MDD compared with the normal control. e A dot plot showing expression levels of the filtered genes in each region with filtered gene sets of hippocampal subregions of patients with MDD compared with the normal control. The data are represented as dot plot; samples included n = 6 for normal control and n = 3 for with MDD in each group. An unpaired two-tailed t-test was used for statistical analysis. f A dot plot showing normalized enrichment score and P value of patients with MDD and control dentate gyrus using a GSEA of Reactome downstream signaling of activated FGFR in the hippocampal subregions. g Representative images showing the expression of FGFR1 in human patients with MDD and normal control dentate gyrus. Scale bar, 200 μm (inset 50 μm). h A quantification of the data shown in g. The data are represented as means ± s.e.m.; samples included n = 9 for normal control and n = 6 for with MDD in each group. An unpaired two-tailed t-test was used for statistical analysis. (t(13) = 12.81, P < 0.0001). ****P < 0.0001.