Fig. 6: Metabolic phenotypes of preadipocyte-specific Pak4-KO mice under normal chow diet feeding.
From: PAK4 phosphorylates cyclin-dependent kinase 2 to promote the G1/S transition during adipogenesis
a A schematic representation of preadipocyte-specific Pak4 ablation using Pdgfrb-Cre mice. b Western blot analysis of primary preadipocytes isolated from WT and Pak4-KO mice to assess PAK4 expression. c Representative images of Pak4-KO and WT littermates (top), along with gross morphology of brown adipose tissue (BAT), inguinal adipose tissue (IAT) and EAT (bottom) at 14 weeks of age. d Microscopic analysis of EAT was conducted using hematoxylin and eosin (H&E) staining and BODIPY immunostaining, with adipocyte size in the H&E sections quantified. e Fat content in mice was measured using an NMR analyzer. f PAK4-mediated phosphorylation of CDK2 and the expression of adipogenic markers were examined in EATs from both WT and Pak4-KO mice. g, h SVCs isolated from EAT of WT and Pak4-KO mice were differentiated into mature adipocytes for 8 days, and adipogenic marker protein levels and lipid accumulation were assessed. Values are mean ± s.d. *P < 0.05, **P < 0.01.
