Fig. 7: PAK4 interaction with CDK2 in human adipocytes.
From: PAK4 phosphorylates cyclin-dependent kinase 2 to promote the G1/S transition during adipogenesis
a SVCs isolated from mesenteric fat tissues of human subjects were transfected with PAK4 siRNA, followed by differentiation into mature adipocytes with MDI cocktail for the indicated durations. Protein levels of adipogenic markers were assessed via western blotting. b After 6 days of differentiation, Oil Red O staining and BODIPY immunostaining were conducted to evaluate lipid accumulation. c After 1 day of differentiation, the interaction between CDK2 and C/EBPβ was analyzed using PLA (n = 6 per group). d Genotype tissue expression (GTEx) analysis of human adipose tissues (n = 355).
