Fig. 2: EPA reduces CD44 expression in IL-1α-treated porcine chondrocytes and in human OA chondrocytes. | Experimental & Molecular Medicine

Fig. 2: EPA reduces CD44 expression in IL-1α-treated porcine chondrocytes and in human OA chondrocytes.

From: Eicosapentaenoic acid restores inflammation-induced changes in chondrocyte mechanics by suppressing the NF-κB p65/CD44 signaling pathway and attenuates osteoarthritis

Fig. 2

a–c PCA plot of RNA-seq samples (a) and volcano maps of DEGs generated from porcine chondrocytes (b and c). Values of FDR <0.05 and log2|fold change| >1 were accepted as significant. d–h Gene expression levels of CXCL2 (d), CXCL8 (e), MMP3 (f), MMP12 (g) and PTGS2 (h) in porcine chondrocytes. i KEGG bubble plot showing enriched pathways across experimental and human OA datasets. j, k GSEA plots illustrating directional regulation of representative pathways. l Heatmap of DEGs in the ECM–receptor interaction pathway in porcine chondrocytes. m–r Gene expression level, representative images of western blot gel and protein expression level of CD44 in porcine chondrocytes and human OA chondrocytes. s–w Representative co-immunostaining images (s) and fluorescence intensity (t–w) of F-actin and CD44 in porcine chondrocytes and human OA chondrocytes. Scale bar, 20 μm. Data are presented as mean ± s.d. n = 3 (d–h, m, o and p), 4 (r), 100 (t and u) and 50 (v and w). Statistical analysis was performed using one-way ANOVA with Tukey’s multiple-comparisons test in d–h, m, o, t and u, or unpaired Student’s t-test in p, r, v and w. *P < 0.05, **P < 0.01, ***P < 0.001.

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